Parametric imaging of the local attenuation coefficient in human axillary lymph nodes assessed using optical coherence tomography
Identifieur interne : 004050 ( Main/Exploration ); précédent : 004049; suivant : 004051Parametric imaging of the local attenuation coefficient in human axillary lymph nodes assessed using optical coherence tomography
Auteurs : Loretta Scolaro [Australie] ; Robert A. Mclaughlin [Australie] ; Blake R. Klyen [Australie] ; Benjamin A. Wood [Australie] ; Peter D. Robbins [Australie] ; Christobel M. Saunders [Australie] ; Steven L. Jacques [États-Unis] ; David D. Sampson [Australie]Source :
- Biomedical Optics Express [ 2156-7085 ] ; 2012.
Abstract
We report the use of optical coherence tomography (OCT) to determine spatially localized optical attenuation coefficients of human axillary lymph nodes and their use to generate parametric images of lymphoid tissue. 3D-OCT images were obtained from excised lymph nodes and optical attenuation coefficients were extracted assuming a single scattering model of OCT. We present the measured attenuation coefficients for several tissue regions in benign and reactive lymph nodes, as identified by histopathology. We show parametric images of the measured attenuation coefficients as well as segmented images of tissue type based on thresholding of the attenuation coefficient values. Comparison to histology demonstrates the enhancement of contrast in parametric images relative to OCT images. This enhancement is a step towards the use of OCT for
Url:
DOI: 10.1364/BOE.3.000366
PubMed: 22312589
PubMed Central: 3269853
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en"><p>We report the use of optical coherence tomography (OCT) to determine spatially localized optical attenuation coefficients of human axillary lymph nodes and their use to generate parametric images of lymphoid tissue. 3D-OCT images were obtained from excised lymph nodes and optical attenuation coefficients were extracted assuming a single scattering model of OCT. We present the measured attenuation coefficients for several tissue regions in benign and reactive lymph nodes, as identified by histopathology. We show parametric images of the measured attenuation coefficients as well as segmented images of tissue type based on thresholding of the attenuation coefficient values. Comparison to histology demonstrates the enhancement of contrast in parametric images relative to OCT images. This enhancement is a step towards the use of OCT for <italic>in situ</italic>
assessment of lymph nodes.</p>
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